Health and Synthetic Biology VIII
Translation by I. A. Martínez
Health and Synthetic Biology I - Tackling infections - Bacteriophages and Quorum sensing vs cholera
Health and Synthetic Biology II - Vaccines and drugs - Vaccines
Health and Synthetic Biology III - Vaccines and drugs - Discovering new drugs
Health and Synthetic Biology IV - Vaccines and drugs - Expanding the genetic code
Health and Synthetic Biology V - Vaccines and drugs -Metabolic engineering
Health and Synthetic Biology VI - Health and iGEM - Bacteria, parasites and yeasts I
Health and Synthetic Biology VII - Health and iGEM - Bacteria, parasites and yeasts II
Health and Synthetic Biology VIII - Health and iGEM - Bacteria, parasites and yeasts III
Health and Synthetic Biology IX - Health and iGEM - Bacteria, parasites and yeasts IV and Gene therapy, non-infectious diseases and mammalian cells chassis
Health and iGEM
Bacteria, parasites and yeasts
Biosensors and vectors - The BIOTEC Dresden 2010 team devised an E. coli chassis capable of producing a transcriptional response against a clinically relevant stimulus. This biosensor could detect the presence of CD33 and other markers of leukemia, also allowing for the amplification of the signal. Basically, the system consists of antibodies that bind to the antigen of interest, then, a Protein A-Luxl chimera joins them and starts producing AHL; the increase in the concentration of AHL would subsequently begin to produce a transcriptional response of a reporter gene (GPF, for example) on the E. coli adapted to respond to this quorum sensing signal.
Another clinically relevant biosensor was developed by team Waterloo 2010 and consisted on an E. coli chassis capable of producing RFP in the presence of quorum sensing signals from Staphylococcus aureus. Also, team Imperial College 2010 devised a biosensor on a chassis of Bacillus subtilis capable of detecting the parasite Schistosoma through an artificial two-component signaling system that is activated by a peptide that produced after the degradation of a surface protein in B. subtilis cell; the degradation of this surface protein is mediated by a Schistosoma protease.
Moreover, a particularly interesting application of bacterial chassis is their use as vectors. The SupBiotech-Paris 2009 team developed a “double vector” system for gene therapy in which a bacterial chassis is responsible to recognize the tissue of interest; within these bacteria would be a bacteriophage chassis –which has been modified to contain some human cell receptors- that recognizes the specific type of cell within the tissue and finally release the genetic-therapeutic contents in them.